tuj 1 mouse primary antibody  (R&D Systems)

Journal: Frontiers in Immunology

Article Title: Microglia/Macrophages and CD4 + CD25 + T Cells Enhance the Ability of Injury-Activated Lymphocytes to Reduce Traumatic Optic Neuropathy In Vitro

doi: 10.3389/fimmu.2021.687898

Figure Lengend Snippet: Effect of lymphocyte co-culture on retinal ganglion cell (RGC) survival and macrophage infiltration. (A) Representative images of flat-mounted retinas showing β-III tubulin-positive RGCs and ED1-positive microglia/macrophages. Scale bar, 50 µm. (B) Quantification of RGCs showed that the co-culture of activated lymphocytes increased the RGCs compared to control lymphocytes co-culture. (C) Quantification of microglia/macrophages showed dramatic augmentation in the co-culture of activated lymphocytes compared to other culture conditions. Data are presented as means ± SEM (n=4-6 of each group). **** P < 0.0001. The number of retinas in each group was 4-6.

Article Snippet: One half was immunostained with primary antibody against mouse anti-βIII-tubulin (TUJ-1, 1:400, Babco, Richmond, CA, USA), and the other half with mouse anti-ED1 (1:200, Serotec, Oxford) followed by incubation with a Cy3-conjugated secondary anti-mouse antibody (1:400, Jackson ImmunoResearch, West Grove, PA, USA) and FITC-conjugated secondary anti-mouse antibody (1:400 Sigma) as secondary antibodies ( , , ).

Techniques: Co-Culture Assay, Control

Journal: American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons

Article Title: Severing corneal nerves in one eye induces sympathetic loss of immune privilege and promotes rejection of future corneal allografts placed in either eye

doi: 10.1111/ajt.13240

Figure Lengend Snippet: A) Cornea from a normal BALB/c mouse; B) cornea collected from a BALB/c mouse 96 hr after being scored with shallow360° incisions created by a 2.0 mm trephine. Arrows indicate site of circular corneal incision. C) Cornea collected 96 hr after being scored with an “X” shaped incision. Corneal nerves were stained with rabbit anti-mouse β tubulin III primary antibody (TUJ1) IgG (Covance, Richmond, CA) and were viewed by confocal microscopy. “C” indicates center of cornea. D) normal cornea. E) Cornea with circular incision (stained with India ink). F) Cornea with “X-shaped” incision (stained with Evan’s blue).

Article Snippet: Corneas were incubated with rabbit anti-mouse β III tubulin primary antibody (TUJ 1 ) IgG (Covance, Richmond, CA), washed in PBS, corneas, and incubated with Propidium Iodide and secondary antibody (Alexa 488 goat anti-rabbit IgG, Invitrogen Carlsbad, CA) for 2.5 hours at room temperature.

Techniques: Staining, Confocal Microscopy

Journal: American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons

Article Title: Severing corneal nerves in one eye induces sympathetic loss of immune privilege and promotes rejection of future corneal allografts placed in either eye

doi: 10.1111/ajt.13240

Figure Lengend Snippet: A) Cornea from a normal BALB/c mouse; B) cornea collected from a BALB/c mouse 96 hr after being scored with shallow360° incisions created by a 2.0 mm trephine. Arrows indicate site of circular corneal incision. C) Cornea collected 96 hr after being scored with an “X” shaped incision. Corneal nerves were stained with rabbit anti-mouse β tubulin III primary antibody (TUJ1) IgG (Covance, Richmond, CA) and were viewed by confocal microscopy. “C” indicates center of cornea. D) normal cornea. E) Cornea with circular incision (stained with India ink). F) Cornea with “X-shaped” incision (stained with Evan’s blue).

Article Snippet: Corneal nerves were stained with rabbit anti-mouse β tubulin III primary antibody (TUJ 1 ) IgG (Covance, Richmond, CA) and were viewed by confocal microscopy.

Techniques: Staining, Confocal Microscopy